Caspase 3 anticorps (AA 207-220)

N° du produit ABIN3030299

L’anticorps Lapin Polyclonal anti-Caspase 3 a été validé pour WB. Il convient pour détecter Caspase 3 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Caspase 3 anticorps (AA 207-220) (ABIN3030299)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Caspase 3 anticorps

Épitope: AA 207-220

Purification: Antigen affinity

Immunogène: Amino acids 207-220 (RNSKDGSWFIQSLC-human) were used as the immunogen for this Caspase-3 antibody (100% mouse homology). This amino acid sequence is from the small subunit.

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase-3

Sujet: Cysteine-aspartic acid protease 3, also known as Apopain, CPP32, CPP32B, and YAMA, is a member of the cysteine-aspartic acid protease family. Western blot analysis of three cell lines revealed the prominent CASP3 band at 32 kD and 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive Caspase-3 by human kidney cells offered some resistance to inducers of apoptosis, and CASP3 accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alternative splicing of this gene results in two transcript variants that encode the same protein. Orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that the protein is important for the initiation of apoptotic cell death.

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation